Coupled replication-translation methods and kits for protein synthesis

Ying Wu; Lyubov A. Ryabova; Oleg V. Kurnasov; Igor Morozov; Viktor I. Ugarov; Elena V. Volianik; Alexander B. Chetverin; David Zhang; Fred R. Kramer; Alexander S. Spirin

Coupled replication-translation methods and kits for protein synthesis

Ying Wu (Inventor), Lyubov A. Ryabova (Inventor), Oleg V. Kurnasov (Inventor), Igor Morozov (Inventor), Viktor I. Ugarov (Inventor), Elena V. Volianik (Inventor), Alexander B. Chetverin (Inventor), David Zhang (Inventor), Fred R. Kramer (Inventor), Alexander S. Spirin (Inventor)

Research output: Patent

Abstract

Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.

Original language	English
Patent number	US5556769
Priority date	17/09/96
Filing date	23/08/94
Publication status	Published - 1996

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Coupled replication-translation methods and kits for protein synthesis. / Wu, Ying (Inventor); Ryabova, Lyubov A. (Inventor); Kurnasov, Oleg V. (Inventor); Morozov, Igor (Inventor); Ugarov, Viktor I. (Inventor); Volianik, Elena V. (Inventor); Chetverin, Alexander B. (Inventor); Zhang, David (Inventor); Kramer, Fred R. (Inventor); Spirin, Alexander S. (Inventor).

Patent No.: US5556769.

Research output: Patent

Wu, Ying (Inventor) ; Ryabova, Lyubov A. (Inventor) ; Kurnasov, Oleg V. (Inventor) ; Morozov, Igor (Inventor) ; Ugarov, Viktor I. (Inventor) ; Volianik, Elena V. (Inventor) ; Chetverin, Alexander B. (Inventor) ; Zhang, David (Inventor) ; Kramer, Fred R. (Inventor) ; Spirin, Alexander S. (Inventor). / Coupled replication-translation methods and kits for protein synthesis. Patent No.: US5556769.

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title = "Coupled replication-translation methods and kits for protein synthesis",

abstract = "Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.",

author = "Ying Wu and Ryabova, {Lyubov A.} and Kurnasov, {Oleg V.} and Igor Morozov and Ugarov, {Viktor I.} and Volianik, {Elena V.} and Chetverin, {Alexander B.} and David Zhang and Kramer, {Fred R.} and Spirin, {Alexander S.}",

year = "1996",

language = "English",

type = "Patent",

note = "US5556769",

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AU - Wu, Ying

AU - Ryabova, Lyubov A.

AU - Kurnasov, Oleg V.

AU - Morozov, Igor

AU - Ugarov, Viktor I.

AU - Volianik, Elena V.

AU - Chetverin, Alexander B.

AU - Zhang, David

AU - Kramer, Fred R.

AU - Spirin, Alexander S.

PY - 1996

Y1 - 1996

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AB - Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.

UR - https://patentimages.storage.googleapis.com/0c/a6/d1/971b4b7daaed97/US5556769.pdf

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