Coupled replication-translation methods and kits for protein synthesis

Ying Wu; Lyubov A. Ryabova; Oleg V. Kurnasov; Igor Morozov; Viktor I. Ugarov; Elena V. Volianik; Alexander B. Chetverin; David Zhang; Fred R. Kramer; Alexander S. Spirin

Coupled replication-translation methods and kits for protein synthesis

Ying Wu (Inventor)
, Lyubov A. Ryabova (Inventor)
, Oleg V. Kurnasov (Inventor)
, Igor Morozov (Inventor)
, Viktor I. Ugarov (Inventor)
, Elena V. Volianik (Inventor)
, Alexander B. Chetverin (Inventor)
, David Zhang (Inventor)
, Fred R. Kramer (Inventor)
, Alexander S. Spirin (Inventor)

Research output: Patent

Abstract

Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.

Original language	English
Patent number	US5556769A
IPC	C12P 21/02,C07H 21/04
Priority date	24/09/92
Filing date	23/08/94
Publication status	Published - 17 Sept 1996

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@misc{84a92bce98224f20945eb2de562bc479,

title = "Coupled replication-translation methods and kits for protein synthesis",

abstract = "Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.",

author = "Ying Wu and Ryabova, \{Lyubov A.\} and Kurnasov, \{Oleg V.\} and Igor Morozov and Ugarov, \{Viktor I.\} and Volianik, \{Elena V.\} and Chetverin, \{Alexander B.\} and David Zhang and Kramer, \{Fred R.\} and Spirin, \{Alexander S.\}",

year = "1996",

month = sep,

day = "17",

language = "English",

type = "Patent",

note = "US5556769A; C12P 21/02,C07H 21/04",

}

TY - PAT

T1 - Coupled replication-translation methods and kits for protein synthesis

AU - Wu, Ying

AU - Ryabova, Lyubov A.

AU - Kurnasov, Oleg V.

AU - Morozov, Igor

AU - Ugarov, Viktor I.

AU - Volianik, Elena V.

AU - Chetverin, Alexander B.

AU - Zhang, David

AU - Kramer, Fred R.

AU - Spirin, Alexander S.

PY - 1996/9/17

Y1 - 1996/9/17

N2 - Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.

AB - Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.

M3 - Patent

M1 - US5556769A

Y2 - 1994/08/23

ER -

Coupled replication-translation methods and kits for protein synthesis

Abstract

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