Coupled replication-translation methods and kits for protein synthesis

Ying Wu (Inventor), Lyubov A. Ryabova (Inventor), Oleg V. Kurnasov (Inventor), Igor Morozov (Inventor), Viktor I. Ugarov (Inventor), Elena V. Volianik (Inventor), Alexander B. Chetverin (Inventor), David Zhang (Inventor), Fred R. Kramer (Inventor), Alexander S. Spirin (Inventor)

Research output: Patent

Abstract

Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.
Original languageEnglish
Patent numberUS5556769
Priority date17/09/96
Filing date23/08/94
Publication statusPublished - 1996

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Messenger RNA
Proteins
Protein Biosynthesis
RNA
DNA
recombinant RNA

Cite this

Wu, Y., Ryabova, L. A., Kurnasov, O. V., Morozov, I., Ugarov, V. I., Volianik, E. V., ... Spirin, A. S. (1996). Coupled replication-translation methods and kits for protein synthesis. (Patent No. US5556769).

Coupled replication-translation methods and kits for protein synthesis. / Wu, Ying (Inventor); Ryabova, Lyubov A. (Inventor); Kurnasov, Oleg V. (Inventor); Morozov, Igor (Inventor); Ugarov, Viktor I. (Inventor); Volianik, Elena V. (Inventor); Chetverin, Alexander B. (Inventor); Zhang, David (Inventor); Kramer, Fred R. (Inventor); Spirin, Alexander S. (Inventor).

Patent No.: US5556769. Aug 23, 1994.

Research output: Patent

Wu, Y, Ryabova, LA, Kurnasov, OV, Morozov, I, Ugarov, VI, Volianik, EV, Chetverin, AB, Zhang, D, Kramer, FR & Spirin, AS Aug. 23 1994, Coupled replication-translation methods and kits for protein synthesis, Patent No. US5556769.
Wu Y, Ryabova LA, Kurnasov OV, Morozov I, Ugarov VI, Volianik EV et al, inventors. Coupled replication-translation methods and kits for protein synthesis. US5556769. 1996.
Wu, Ying (Inventor) ; Ryabova, Lyubov A. (Inventor) ; Kurnasov, Oleg V. (Inventor) ; Morozov, Igor (Inventor) ; Ugarov, Viktor I. (Inventor) ; Volianik, Elena V. (Inventor) ; Chetverin, Alexander B. (Inventor) ; Zhang, David (Inventor) ; Kramer, Fred R. (Inventor) ; Spirin, Alexander S. (Inventor). / Coupled replication-translation methods and kits for protein synthesis. Patent No.: US5556769. Aug 23, 1994.
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abstract = "Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.",
author = "Ying Wu and Ryabova, {Lyubov A.} and Kurnasov, {Oleg V.} and Igor Morozov and Ugarov, {Viktor I.} and Volianik, {Elena V.} and Chetverin, {Alexander B.} and David Zhang and Kramer, {Fred R.} and Spirin, {Alexander S.}",
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AU - Ryabova, Lyubov A.

AU - Kurnasov, Oleg V.

AU - Morozov, Igor

AU - Ugarov, Viktor I.

AU - Volianik, Elena V.

AU - Chetverin, Alexander B.

AU - Zhang, David

AU - Kramer, Fred R.

AU - Spirin, Alexander S.

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AB - Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.

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