Abstract
Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.
Original language | English |
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Patent number | US5556769A |
IPC | C12P 21/02,C07H 21/04 |
Priority date | 24/09/92 |
Filing date | 23/08/94 |
Publication status | Published - 17 Sept 1996 |