Conformational flexibility influences structure–function relationships in tyrosyl protein sulfotransferase-2

W. Singh, T. G. Karabencheva-Christova, G. W. Black, Olivier Sparagano, C. Z. Christov

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Abstract

Tyrosine sulfation is a very important posttranslational modification of proteins. It is catalyzed by tyrosylprotein sulfotransferase and recently became increasingly important for biomedicine and pharmacy. An important insight about structure–activity relationships of human tyrosylprotein sulfotransferase has been received by elucidating the crystal structure, but there is still no understanding about how conformational flexibility and dynamics which are fundamental protein properties influence structure–function relationships of the enzyme. In order to provide this missing but crucially important knowledge we performed a comprehensive atomistic molecular dynamics study which revealed that (i) the conformational flexibility influences sensitively key structural determinants and interactions between the enzyme, the substrate and the cofactor; (ii) a more open conformation adopted by the substrate for binding in TPST 2; (iii) the mutations of key residues related with catalysis and binding change alter the enzyme structure and influence important interactions between the enzyme, the cofactor and the substrate.
Original languageEnglish
Pages (from-to)11344-11352
JournalRSC Advances
Volume6
DOIs
Publication statusPublished - 18 Jan 2016

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Enzymes
Proteins
Substrates
Coenzymes
Catalysis
Tyrosine
Conformations
Molecular dynamics
Crystal structure
protein-tyrosine sulfotransferase
thymidylyl(3'-5')-5'-thio-5'-deoxythymidine

Bibliographical note

The full text is also available from: http://dx.doi.org/10.1039/C5RA25365A
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Cite this

Singh, W., Karabencheva-Christova, T. G., Black, G. W., Sparagano, O., & Christov, C. Z. (2016). Conformational flexibility influences structure–function relationships in tyrosyl protein sulfotransferase-2. RSC Advances, 6, 11344-11352. https://doi.org/10.1039/C5RA25365A

Conformational flexibility influences structure–function relationships in tyrosyl protein sulfotransferase-2. / Singh, W.; Karabencheva-Christova, T. G.; Black, G. W.; Sparagano, Olivier; Christov, C. Z.

In: RSC Advances, Vol. 6, 18.01.2016, p. 11344-11352.

Research output: Contribution to journalArticle

Singh, W, Karabencheva-Christova, TG, Black, GW, Sparagano, O & Christov, CZ 2016, 'Conformational flexibility influences structure–function relationships in tyrosyl protein sulfotransferase-2' RSC Advances, vol. 6, pp. 11344-11352. https://doi.org/10.1039/C5RA25365A
Singh, W. ; Karabencheva-Christova, T. G. ; Black, G. W. ; Sparagano, Olivier ; Christov, C. Z. / Conformational flexibility influences structure–function relationships in tyrosyl protein sulfotransferase-2. In: RSC Advances. 2016 ; Vol. 6. pp. 11344-11352.
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