Concurrent resistance and aerobic exercise stimulates both myofibrillar and mitochondrial protein synthesis in sedentary middle-aged men

Cheyne E. Donges, Nicholas A. Burd, Rob Duffield, Greg C. Smith, Daniel W. D. West, Michael J. Short, Richard Mackenzie, Lindsay D. Plank, Peter R. Shepherd, Stuart M. Phillips, Johann A. Edge

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67 Citations (Scopus)

Abstract

We determined myofibrillar and mitochondrial protein fractional synthesis rates (FSR), intramuscular signaling protein phosphorylation, and mRNA expression responses after isolated bouts of resistance exercise (RE), aerobic exercise (AE), or in combination [termed concurrent exercise (CE)] in sedentary middle-aged men. Eight subjects (age   53.3   1.8 yr;
body mass index   29.4   1.4 kg·m2) randomly completed 8   8 leg
extension repetitions at 70% of one repetition-maximum, 40 min of
cycling at 55% peak aerobic power output (AE), or (consecutively)
50% of the RE and AE trials (CE). Biopsies were obtained (during a
primed, constant infusion of L-[ring-13C6]phenylalanine) while fasted,
and at 1 and 4 h following postexercise ingestion of 20 g of protein.
All trials increased mitochondrial FSR above fasted rates (RE  
1.3-fold; AE   1.5; CE   1.4; P   0.05), although only CE (2.2) and
RE (1.8) increased myofibrillar FSR (P   0.05). At 1 h postexercise,
phosphorylation of Akt on Ser473 (CE   7.7; RE   4.6) and Thr308
(CE   4.4; RE   2.9), and PRAS40 on Thr246 (CE   3.8; AE   2.5)
increased (P   0.05), with CE greater than AE for Akt Ser473-Thr308
and greater than RE for PRAS40 (P   0.05). Despite increased
phosphorylation of Akt-PRAS40, phosphorylation of mammalian target
of rapamycin (Ser2448) remained unchanged (P   0.05), while
rpS6 (Ser235/236) increased only in RE (10.4) (P   0.05). CE and AE
both resulted in increased peroxisome proliferator receptor-  coactivator
1-  (PGC1 ) expression at 1 h (CE   2.9; AE   2.8; P   0.05) and 4
h (CE   2.6; AE   2.4) and PGC1  expression at 4 h (CE   2.1; AE  
2.6; P   0.05). These data suggest that CE-induced acute stimulation of
myofibrillar and mitochondrial FSR, protein signaling, and mRNA expression are equivalent to either isolate mode (RE or AE). These results
occurred without an interference effect on muscle protein subfractional
synthesis rates, protein signaling, or mRNA expression.
Original languageEnglish
Pages (from-to)1992-2001
Number of pages10
JournalJournal of Applied Physiology
Volume112
Issue number12
Early online date5 Apr 2012
DOIs
Publication statusPublished - 15 Jun 2012
Externally publishedYes

Keywords

  • untrained adults
  • muscle protein synthesis
  • anabolic protein signaling
  • gene expression

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