Comparative assessment of the acute effects of whey, rice and potato protein isolate intake on markers of glycaemic regulation and appetite in healthy males using a randomised study design

Helena Tiekou Lorinczova, Sanjoy Deb, Gulshanara Begum, Derek Renshaw, Mohammed Gulrez Zariwala

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    6 Citations (Scopus)
    33 Downloads (Pure)


    Global protein consumption has been increasing for decades due to changes in demographics and consumer shifts towards higher protein intake to gain health benefits in performance nutrition and appetite regulation. Plant-derived proteins may provide a more environmentally sustainable alternative to animal-derived proteins. This study, therefore, aimed to investigate, for the first time, the acute effects on glycaemic indices, gut hormones, and subjective appetite ratings of two high-quality, plant-derived protein isolates (potato and rice), in comparison to a whey protein isolate in a single-blind, triple-crossover design study with nine male participants (30.8 ± 9.3 yrs). Following a 12 h overnight fast, participants consumed an equal volume of the three isocaloric protein shakes on different days, with at least a one-week washout period. Glycaemic indices and gut hormones were measured at baseline, then at 30, 60, 120, 180 min at each visit. Subjective palatability and appetite ratings were measured using visual analogue scales (VAS) over the 3 h, at each visit. This data showed significant differences in insulin secretion with an increase in whey (+141.8 ± 35.1 pmol/L; p = 0.011) and rice (−64.4 ± 20.9 pmol/L; p = 0.046) at 30 min compared to potato protein. A significantly larger total incremental area under the curve (iAUC) was observed with whey versus potato and rice with p < 0.001 and p = 0.010, respectively. There was no significant difference observed in average appetite perception between the different proteins. In conclusion, this study suggests that both plant-derived proteins had a lower insulinaemic response and improved glucose maintenance compared to whey protein.

    Original languageEnglish
    Article number2157
    Issue number7
    Publication statusPublished - 23 Jun 2021

    Bibliographical note

    Funding Information:
    We gratefully acknowledge the time and dedication of all participants who participated in this study. We thank Davisco Foods International, Inc., Eden Prairie, MN, USA, for providing us samples of Instantized BiPRO?? and Axiom Foods/Growing Naturals, Inc., Los Angeles, CA, USA, for providing us samples of Organic Oryzatein?? Silk 90. We would like to acknowledge all colleagues from The University of Westminster who contributed support and expertise to this study. We thank Bradley Elliot for his assistance and training with the cannulation protocol and Helen Lloyd for her technical assistance.

    Publisher Copyright:
    © 2021 by the authors. Licensee MDPI, Basel, Switzerland.


    • Animal-derived proteins
    • Appetite
    • GLP-1
    • Glucose homeostasis
    • Insulin
    • Plant-derived proteins
    • Potato protein
    • Protein
    • Rice protein
    • Type 2 diabetes mellitus (T2DM)
    • Whey protein

    ASJC Scopus subject areas

    • Food Science
    • Nutrition and Dietetics


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