Circadian Regulation of Plant Immunity to Pathogens

Robert A. Ingle, Laura C. Roden

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The plant circadian clock primes the immune response of Arabidopsis thaliana to infection with the bacterial pathogen Pseudomonas syringae pv tomato DC3000 (Pst DC3000) such that there is a more robust response during the subjective day than subjective night. Thus Pst DC3000 growth in plants infected with the same initial titre of bacteria varies depending on the time of day of infection (Bhardwaj et al., PLoS One 6: e26968, 2011; Zhang et al., PLoS Pathog 9:e1003370, 2013). We describe here a protocol for assaying bacterial leaf titres following pressure infiltration or spray inoculation of Arabidopsis thaliana with Pst DC3000. We also describe a method for assaying plant susceptibility to infection with the necrotrophic fungal pathogen Botrytis cinerea. These methods can be used in studying the circadian clock regulation of signal transduction pathways controlling plant defense responses.
Original languageEnglish
Title of host publicationPlant Circadian Networks
PublisherHumana Press
Pages273-283
Number of pages11
ISBN (Electronic)978-1-4939-0700-7
ISBN (Print)978-1-4939-0699-4
DOIs
Publication statusPublished - 8 Apr 2014
Externally publishedYes

Fingerprint

Pseudomonas syringae
immunity
tomatoes
circadian rhythm
pathogens
Arabidopsis thaliana
infection
Botrytis cinerea
signal transduction
immune response
plant growth
bacteria
methodology
leaves

Cite this

Ingle, R. A., & Roden, L. C. (2014). Circadian Regulation of Plant Immunity to Pathogens. In Plant Circadian Networks (pp. 273-283). Humana Press. https://doi.org/10.1007/978-1-4939-0700-7_18

Circadian Regulation of Plant Immunity to Pathogens. / Ingle, Robert A.; Roden, Laura C.

Plant Circadian Networks. Humana Press, 2014. p. 273-283.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ingle, RA & Roden, LC 2014, Circadian Regulation of Plant Immunity to Pathogens. in Plant Circadian Networks. Humana Press, pp. 273-283. https://doi.org/10.1007/978-1-4939-0700-7_18
Ingle RA, Roden LC. Circadian Regulation of Plant Immunity to Pathogens. In Plant Circadian Networks. Humana Press. 2014. p. 273-283 https://doi.org/10.1007/978-1-4939-0700-7_18
Ingle, Robert A. ; Roden, Laura C. / Circadian Regulation of Plant Immunity to Pathogens. Plant Circadian Networks. Humana Press, 2014. pp. 273-283
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