Characterization of lactic acid bacteria and other gut bacteria in pigs by a macroarraying method

Narut Thanantong, Sandra Edwards, Olivier A.E. Sparagano

Research output: Chapter in Book/Report/Conference proceedingConference proceeding

7 Citations (Scopus)

Abstract

Lactic acid bacteria (LAB) consist of many genera, Gram-positive, and nonspore-forming micro-organisms; some members being used as probiotics while some others have negative effects on pig health. Bacterial species in the gastrointestinal tract can produce antibacterial substances, reduce serum cholesterol in their host, or can be responsible for growth reduction, diarrhea, and intestinal epithelial damage. It is therefore important for the pig industry to evaluate the impact of food and farm management on the presence of "good" or "bad" bacteria and the risk for consumers. This articles focuses on the molecular identification of gut microflora species following different diets given to pigs in UK and correlating the data on growth, health, and welfare. First of all, pig feces were individually collected from sows before and after farrowing and also from piglets before and after weaning over several months. Bacteria colonies were grown on MRS agar plates from feces and DNA was extracted (QIAamp DNA stool kit) and amplified using 16S rDNA (27f and 519r) primers. DNA sequencing and sequence alignment allowed us to identify species-specific zones, which were used as probes in a macroarray system also known as reverse line blot hybridization. Some probes were found to be species specific for the following species: Lactobacillus acidophilus, L. animalis, L. gallinarum, L. kitasanotis, L salivarius, Streptococcus alactolyticus, S. hyointestinalis, and Sarcina ventriculi. Actual studies are now focusing on the impact of diets of the microflora in different gut parts and at different stages of the animal's life.

Original languageEnglish
Title of host publicationImpact of Emerging Zoonotic Diseases on Animal Health
Subtitle of host publication8th Biennial Conference of the Society for Tropical Veterinary Medicine
PublisherWiley
Pages276-279
Number of pages4
Volume1081
Edition1
ISBN (Print)1573316377, 9781573316378
DOIs
Publication statusPublished - 1 Jan 2006
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1081
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Diet
  • Gut
  • Lactobacillus
  • Pig
  • RLB

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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  • Cite this

    Thanantong, N., Edwards, S., & Sparagano, O. A. E. (2006). Characterization of lactic acid bacteria and other gut bacteria in pigs by a macroarraying method. In Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine (1 ed., Vol. 1081, pp. 276-279). (Annals of the New York Academy of Sciences; Vol. 1081). Wiley. https://doi.org/10.1196/annals.1373.037