Characterization and discrimination of three Theileria parva stabilates involved in East Coast fever vaccination

O. A.E. Sparagano, O. Zanaa, N. Ambrose

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Three vaccine stabilates of Theileria parva, of which sporozoites are being used against East Coast fever, were characterized by immunological and molecular biology techniques before being used for a national vaccination campaign in Kenya. T. parva Marikebuni stabilates 316 and 3014, and T. parva Lanet were used in this study and were discriminated from other Kenyan field Theileria isolates. IFAT results showed that all the animals were producing antibodies regardless of the stock used. Primers designed on the TPR1 gene sequence were used for PCR and Decamers were used for RAPD. Specific DNA band patterns(1877 bp; 1059 bp, and 433 bp) for the three vaccine stocks were observed. These molecular markers could be used to trace vaccinated animals in Kenya and to identify which isolates are responsible for reactions in animals.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalAnnals of the New York Academy of Sciences
Volume849
Issue number1
DOIs
Publication statusPublished - 1 Jan 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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