Characterisation of recombinant unglycosylated human serum transferrin purified from Saccharomyces cerevisiae

Peter J. Sargent, Sebastien Farnaud, Richard Cammack, Heinz M P Zoller, Robert W. Evans

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Structural identity between a recombinant transferrin mutant (N413Q, N611Q) secreted from Saccharomyces cerevisiae and the native protein was shown by CD analysis and immunodiffusion assays against anti-hSTf. The ability of the recombinant protein to bind iron was confirmed by urea-PAGE and EPR analysis of the iron-saturated protein revealed the characteristic holo-transferrin spectrum, indicating conservation of both iron-binding sites. The integrity of the unglycosylated recombinant protein indicates that such protein could be a valuable tool not only for structure-function characterisation but also crystallisation assays. In addition, the recombinant transferrin was found to be as effective as native transferrin as a growth factor in cell culture medium.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalBioMetals
Volume19
Issue number5
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Fingerprint

transferrin
Transferrin
Yeast
Saccharomyces cerevisiae
Recombinant proteins
Iron
Proteins
Assays
iron
Serum
Recombinant Proteins
recombinant proteins
Binding sites
Saccharomyces cerevisiae Proteins
Cell culture
Urea
Paramagnetic resonance
proteins
Immunodiffusion
Conservation

Keywords

  • Characterisation
  • Iron
  • Recombinant
  • Saccharomyces cerevisiae
  • Transferrin
  • Yeast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Characterisation of recombinant unglycosylated human serum transferrin purified from Saccharomyces cerevisiae. / Sargent, Peter J.; Farnaud, Sebastien; Cammack, Richard; Zoller, Heinz M P; Evans, Robert W.

In: BioMetals, Vol. 19, No. 5, 10.2006, p. 513-519.

Research output: Contribution to journalArticle

Sargent, Peter J. ; Farnaud, Sebastien ; Cammack, Richard ; Zoller, Heinz M P ; Evans, Robert W. / Characterisation of recombinant unglycosylated human serum transferrin purified from Saccharomyces cerevisiae. In: BioMetals. 2006 ; Vol. 19, No. 5. pp. 513-519.
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