Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells

Kosha Mehta, Pamela Greenwell, Derek Renshaw, Mark Busbridge, Mitla Garcia, Sebastien Farnaud, Vinood B Patel

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Iron overload coupled with low hepcidin levels are characteristics of hereditary haemochromatosis. To understand the role of transferrin receptor (TFR) and intracellular iron in hepcidin secretion, Chinese hamster ovary transferrin receptor variant (CHO TRVb-1) cells were used that express iron-response-element-depleted human TFRC mRNA (TFRC∆IRE). Results showed that CHO TRVb-1 cells expressed higher basal levels of cell-surface TFR1 than HepG2 cells (2.2-fold; p < 0.01) and following 5 g/L holotransferrin treatment maintained constitutive over-expression at 24h and 48 h, contrasting the HepG2 cells where the receptor levels significantly declined. Despite this, the intracellular iron content was neither higher than HepG2 cells nor increased over time under basal or holotransferrin-treated conditions. Interestingly, hepcidin secretion in CHO TRVb-1 cells exceeded basal levels at all time-points (p < 0.02) and matched levels in HepG2 cells following treatment. While TFRC mRNA expression showed expected elevation (2h, p < 0.03; 4h; p < 0.05), slc40a1 mRNA expression was also elevated (2 h, p < 0.05; 4 h, p < 0.03), unlike the HepG2 cells. In conclusion, the CHO TRVb-1 cells prevented cellular iron-overload by elevating slc40a1 expression, thereby highlighting its significance in the absence of iron-regulated TFRC mRNA. Furthermore, hepcidin response to holotransferrin treatment was similar to HepG2 cells and resembled the human physiological response.

    Original languageEnglish
    Pages (from-to)110–118
    Number of pages9
    JournalBlood Cells, Molecules, and Diseases
    Volume55
    Issue number2
    Early online date8 May 2015
    DOIs
    Publication statusPublished - Aug 2015

    Fingerprint

    Hepcidins
    Hep G2 Cells
    Iron
    Messenger RNA
    Transferrin Receptors
    Iron Overload
    Hemochromatosis
    Response Elements
    Cricetulus
    holotransferrin
    Ovary

    Keywords

    • Amino Acid Sequence
    • Animals
    • CHO Cells
    • Cation Transport Proteins
    • Cell Membrane
    • Conserved Sequence
    • Cricetinae
    • Cricetulus
    • Gene Expression
    • Hep G2 Cells
    • Hepcidins
    • Humans
    • Intracellular Space
    • Iron
    • Mitochondria
    • Molecular Sequence Data
    • RNA, Messenger
    • Receptors, Transferrin
    • Sequence Alignment
    • Transferrin
    • Journal Article
    • Research Support, Non-U.S. Gov't

    Cite this

    Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells. / Mehta, Kosha; Greenwell, Pamela; Renshaw, Derek; Busbridge, Mark; Garcia, Mitla; Farnaud, Sebastien; Patel, Vinood B.

    In: Blood Cells, Molecules, and Diseases, Vol. 55, No. 2, 08.2015, p. 110–118.

    Research output: Contribution to journalArticle

    Mehta, Kosha ; Greenwell, Pamela ; Renshaw, Derek ; Busbridge, Mark ; Garcia, Mitla ; Farnaud, Sebastien ; Patel, Vinood B. / Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells. In: Blood Cells, Molecules, and Diseases. 2015 ; Vol. 55, No. 2. pp. 110–118.
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    abstract = "Iron overload coupled with low hepcidin levels are characteristics of hereditary haemochromatosis. To understand the role of transferrin receptor (TFR) and intracellular iron in hepcidin secretion, Chinese hamster ovary transferrin receptor variant (CHO TRVb-1) cells were used that express iron-response-element-depleted human TFRC mRNA (TFRC∆IRE). Results showed that CHO TRVb-1 cells expressed higher basal levels of cell-surface TFR1 than HepG2 cells (2.2-fold; p < 0.01) and following 5 g/L holotransferrin treatment maintained constitutive over-expression at 24h and 48 h, contrasting the HepG2 cells where the receptor levels significantly declined. Despite this, the intracellular iron content was neither higher than HepG2 cells nor increased over time under basal or holotransferrin-treated conditions. Interestingly, hepcidin secretion in CHO TRVb-1 cells exceeded basal levels at all time-points (p < 0.02) and matched levels in HepG2 cells following treatment. While TFRC mRNA expression showed expected elevation (2h, p < 0.03; 4h; p < 0.05), slc40a1 mRNA expression was also elevated (2 h, p < 0.05; 4 h, p < 0.03), unlike the HepG2 cells. In conclusion, the CHO TRVb-1 cells prevented cellular iron-overload by elevating slc40a1 expression, thereby highlighting its significance in the absence of iron-regulated TFRC mRNA. Furthermore, hepcidin response to holotransferrin treatment was similar to HepG2 cells and resembled the human physiological response.",
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    AU - Mehta, Kosha

    AU - Greenwell, Pamela

    AU - Renshaw, Derek

    AU - Busbridge, Mark

    AU - Garcia, Mitla

    AU - Farnaud, Sebastien

    AU - Patel, Vinood B

    N1 - Crown Copyright © 2015. Published by Elsevier Inc. All rights reserved.

    PY - 2015/8

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    N2 - Iron overload coupled with low hepcidin levels are characteristics of hereditary haemochromatosis. To understand the role of transferrin receptor (TFR) and intracellular iron in hepcidin secretion, Chinese hamster ovary transferrin receptor variant (CHO TRVb-1) cells were used that express iron-response-element-depleted human TFRC mRNA (TFRC∆IRE). Results showed that CHO TRVb-1 cells expressed higher basal levels of cell-surface TFR1 than HepG2 cells (2.2-fold; p < 0.01) and following 5 g/L holotransferrin treatment maintained constitutive over-expression at 24h and 48 h, contrasting the HepG2 cells where the receptor levels significantly declined. Despite this, the intracellular iron content was neither higher than HepG2 cells nor increased over time under basal or holotransferrin-treated conditions. Interestingly, hepcidin secretion in CHO TRVb-1 cells exceeded basal levels at all time-points (p < 0.02) and matched levels in HepG2 cells following treatment. While TFRC mRNA expression showed expected elevation (2h, p < 0.03; 4h; p < 0.05), slc40a1 mRNA expression was also elevated (2 h, p < 0.05; 4 h, p < 0.03), unlike the HepG2 cells. In conclusion, the CHO TRVb-1 cells prevented cellular iron-overload by elevating slc40a1 expression, thereby highlighting its significance in the absence of iron-regulated TFRC mRNA. Furthermore, hepcidin response to holotransferrin treatment was similar to HepG2 cells and resembled the human physiological response.

    AB - Iron overload coupled with low hepcidin levels are characteristics of hereditary haemochromatosis. To understand the role of transferrin receptor (TFR) and intracellular iron in hepcidin secretion, Chinese hamster ovary transferrin receptor variant (CHO TRVb-1) cells were used that express iron-response-element-depleted human TFRC mRNA (TFRC∆IRE). Results showed that CHO TRVb-1 cells expressed higher basal levels of cell-surface TFR1 than HepG2 cells (2.2-fold; p < 0.01) and following 5 g/L holotransferrin treatment maintained constitutive over-expression at 24h and 48 h, contrasting the HepG2 cells where the receptor levels significantly declined. Despite this, the intracellular iron content was neither higher than HepG2 cells nor increased over time under basal or holotransferrin-treated conditions. Interestingly, hepcidin secretion in CHO TRVb-1 cells exceeded basal levels at all time-points (p < 0.02) and matched levels in HepG2 cells following treatment. While TFRC mRNA expression showed expected elevation (2h, p < 0.03; 4h; p < 0.05), slc40a1 mRNA expression was also elevated (2 h, p < 0.05; 4 h, p < 0.03), unlike the HepG2 cells. In conclusion, the CHO TRVb-1 cells prevented cellular iron-overload by elevating slc40a1 expression, thereby highlighting its significance in the absence of iron-regulated TFRC mRNA. Furthermore, hepcidin response to holotransferrin treatment was similar to HepG2 cells and resembled the human physiological response.

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    KW - Conserved Sequence

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    KW - Cricetulus

    KW - Gene Expression

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    KW - Humans

    KW - Intracellular Space

    KW - Iron

    KW - Mitochondria

    KW - Molecular Sequence Data

    KW - RNA, Messenger

    KW - Receptors, Transferrin

    KW - Sequence Alignment

    KW - Transferrin

    KW - Journal Article

    KW - Research Support, Non-U.S. Gov't

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