Cellular ATP Changes in Acidithiobacillus ferrooxidans Cultures Oxidizing Ferrous Iron and Elemental Sulfur

Eva Pakostova, Martin Mandl, Blanka Omesova Pokorna, Eva Diviskova, Antonin Lojek

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grown
with Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalGeomicrobiology Journal
Volume30
Issue number1
Early online date8 Nov 2012
DOIs
Publication statusPublished - 1 Jan 2013
Externally publishedYes

Bibliographical note

This is an Accepted Manuscript of an article published by Taylor & Francis in Geomicrobiology Journal on 08/11/2012, available online: http://www.tandfonline.com/10.1080/01490451.2011.636790


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Keywords

  • Acidithiobacillus
  • acidophiles
  • ATP assay
  • iron-oxidizing bacteria
  • sulfur-oxidizing bacteria

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