Abstract
Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grown
with Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.
with Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.
Original language | English |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Geomicrobiology Journal |
Volume | 30 |
Issue number | 1 |
Early online date | 8 Nov 2012 |
DOIs | |
Publication status | Published - 1 Jan 2013 |
Externally published | Yes |
Bibliographical note
This is an Accepted Manuscript of an article published by Taylor & Francis in Geomicrobiology Journal on 08/11/2012, available online: http://www.tandfonline.com/10.1080/01490451.2011.636790Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.
Keywords
- Acidithiobacillus
- acidophiles
- ATP assay
- iron-oxidizing bacteria
- sulfur-oxidizing bacteria