Cellular ATP Changes in Acidithiobacillus ferrooxidans Cultures Oxidizing Ferrous Iron and Elemental Sulfur

Eva Pakostova; Martin Mandl; Blanka Omesova Pokorna; Eva Diviskova; Antonin Lojek

doi:10.1080/01490451.2011.636790

Cellular ATP Changes in Acidithiobacillus ferrooxidans Cultures Oxidizing Ferrous Iron and Elemental Sulfur

Eva Pakostova, Martin Mandl, Blanka Omesova Pokorna, Eva Diviskova, Antonin Lojek

Research output: Contribution to journal › Article › peer-review

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Abstract

Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grown
with Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Geomicrobiology Journal
Volume	30
Issue number	1
Early online date	8 Nov 2012
DOIs	https://doi.org/10.1080/01490451.2011.636790
Publication status	Published - 1 Jan 2013
Externally published	Yes

Bibliographical note

This is an Accepted Manuscript of an article published by Taylor & Francis in Geomicrobiology Journal on 08/11/2012, available online: http://www.tandfonline.com/10.1080/01490451.2011.636790

Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

Keywords

Acidithiobacillus
acidophiles
ATP assay
iron-oxidizing bacteria
sulfur-oxidizing bacteria

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title = "Cellular ATP Changes in Acidithiobacillus ferrooxidans Cultures Oxidizing Ferrous Iron and Elemental Sulfur",

abstract = "Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grownwith Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.",

keywords = "Acidithiobacillus, acidophiles, ATP assay, iron-oxidizing bacteria, sulfur-oxidizing bacteria",

author = "Eva Pakostova and Martin Mandl and {Omesova Pokorna}, Blanka and Eva Diviskova and Antonin Lojek",

note = "This is an Accepted Manuscript of an article published by Taylor & Francis in Geomicrobiology Journal on 08/11/2012, available online: http://www.tandfonline.com/10.1080/01490451.2011.636790 Copyright {\textcopyright} and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders. ",

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AU - Pakostova, Eva

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AU - Diviskova, Eva

AU - Lojek, Antonin

N1 - This is an Accepted Manuscript of an article published by Taylor & Francis in Geomicrobiology Journal on 08/11/2012, available online: http://www.tandfonline.com/10.1080/01490451.2011.636790 Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

PY - 2013/1/1

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N2 - Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grownwith Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.

AB - Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grownwith Fe2+ and S0 were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.

KW - Acidithiobacillus

KW - acidophiles

KW - ATP assay

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KW - sulfur-oxidizing bacteria

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Cellular ATP Changes in Acidithiobacillus ferrooxidans Cultures Oxidizing Ferrous Iron and Elemental Sulfur

Abstract

Bibliographical note

Keywords

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