Cellular ATP and biomass of attached and planktonic sulfur-oxidizing Acidithiobacillus ferrooxidans

Eva Pakostova; Martin Mandl; Olli H. Tuovinen

doi:10.1016/j.procbio.2013.07.026

Cellular ATP and biomass of attached and planktonic sulfur-oxidizing Acidithiobacillus ferrooxidans

Eva Pakostova
, Martin Mandl
, Olli H. Tuovinen

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

23 Downloads (Pure)

Abstract

This study demonstrates differences in ATP levels between attached and planktonic cells of Acidithiobacillus ferrooxidans growing with elemental sulfur. A small fraction of 3.7–14.4% of the bacterial cells was attached to the sulfur particles. The highest cell attachment of 14.4% was at the end of the lag phase, decreasing to 3.7% into the latter part of the active growth phase. Therefore, attached cells and their ATP content made a minor contribution to the total culture biomass in the active growth phase. However, the cellular ATP content was 1.01 amol per attached cell and 0.34 amol per planktonic cell. The significantly (P < 0.01) lower ATP content was attributed to sulfur limitation in the planktonic cells. These results suggest that a negligibly small subpopulation may be a link in cooperative interaction whereby sulfur oxidation by attached cells under boundary conditions provides bioavailable substrates to planktonic cells in the population.

Original language	English
Pages (from-to)	1785-1788
Number of pages	4
Journal	Process Biochemistry
Volume	48
Issue number	11
Early online date	12 Aug 2013
DOIs	https://doi.org/10.1016/j.procbio.2013.07.026
Publication status	Published - Nov 2013
Externally published	Yes

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Process Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Process Biochemistry, [48,] [11], (2013)] DOI: 10.1016/j.procbio.2013.07.026

© 2013, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

Acidithiobacillus ferrooxidans
ATP
Attached bacterial cells
Cellular carbon
Sulfur oxidation

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10.1016/j.procbio.2013.07.026

Post-PrintAccepted author manuscript, 645 KBLicence: CC BY-NC-ND

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@article{48903dab182548ba8d32c9debafef556,

title = "Cellular ATP and biomass of attached and planktonic sulfur-oxidizing Acidithiobacillus ferrooxidans",

abstract = "This study demonstrates differences in ATP levels between attached and planktonic cells of Acidithiobacillus ferrooxidans growing with elemental sulfur. A small fraction of 3.7–14.4\% of the bacterial cells was attached to the sulfur particles. The highest cell attachment of 14.4\% was at the end of the lag phase, decreasing to 3.7\% into the latter part of the active growth phase. Therefore, attached cells and their ATP content made a minor contribution to the total culture biomass in the active growth phase. However, the cellular ATP content was 1.01 amol per attached cell and 0.34 amol per planktonic cell. The significantly (P < 0.01) lower ATP content was attributed to sulfur limitation in the planktonic cells. These results suggest that a negligibly small subpopulation may be a link in cooperative interaction whereby sulfur oxidation by attached cells under boundary conditions provides bioavailable substrates to planktonic cells in the population.",

keywords = "Acidithiobacillus ferrooxidans, ATP, Attached bacterial cells, Cellular carbon, Sulfur oxidation",

author = "Eva Pakostova and Martin Mandl and Tuovinen, \{Olli H.\}",

note = "NOTICE: this is the author{\textquoteright}s version of a work that was accepted for publication in Process Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Process Biochemistry, [48,] [11], (2013)] DOI: 10.1016/j.procbio.2013.07.026 {\textcopyright} 2013, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ ",

year = "2013",

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doi = "10.1016/j.procbio.2013.07.026",

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AU - Pakostova, Eva

AU - Mandl, Martin

AU - Tuovinen, Olli H.

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Process Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Process Biochemistry, [48,] [11], (2013)] DOI: 10.1016/j.procbio.2013.07.026 © 2013, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

PY - 2013/11

Y1 - 2013/11

N2 - This study demonstrates differences in ATP levels between attached and planktonic cells of Acidithiobacillus ferrooxidans growing with elemental sulfur. A small fraction of 3.7–14.4% of the bacterial cells was attached to the sulfur particles. The highest cell attachment of 14.4% was at the end of the lag phase, decreasing to 3.7% into the latter part of the active growth phase. Therefore, attached cells and their ATP content made a minor contribution to the total culture biomass in the active growth phase. However, the cellular ATP content was 1.01 amol per attached cell and 0.34 amol per planktonic cell. The significantly (P < 0.01) lower ATP content was attributed to sulfur limitation in the planktonic cells. These results suggest that a negligibly small subpopulation may be a link in cooperative interaction whereby sulfur oxidation by attached cells under boundary conditions provides bioavailable substrates to planktonic cells in the population.

AB - This study demonstrates differences in ATP levels between attached and planktonic cells of Acidithiobacillus ferrooxidans growing with elemental sulfur. A small fraction of 3.7–14.4% of the bacterial cells was attached to the sulfur particles. The highest cell attachment of 14.4% was at the end of the lag phase, decreasing to 3.7% into the latter part of the active growth phase. Therefore, attached cells and their ATP content made a minor contribution to the total culture biomass in the active growth phase. However, the cellular ATP content was 1.01 amol per attached cell and 0.34 amol per planktonic cell. The significantly (P < 0.01) lower ATP content was attributed to sulfur limitation in the planktonic cells. These results suggest that a negligibly small subpopulation may be a link in cooperative interaction whereby sulfur oxidation by attached cells under boundary conditions provides bioavailable substrates to planktonic cells in the population.

KW - Acidithiobacillus ferrooxidans

KW - ATP

KW - Attached bacterial cells

KW - Cellular carbon

KW - Sulfur oxidation

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DO - 10.1016/j.procbio.2013.07.026

M3 - Article

SN - 1359-5113

VL - 48

SP - 1785

EP - 1788

JO - Process Biochemistry

JF - Process Biochemistry

IS - 11

ER -

Cellular ATP and biomass of attached and planktonic sulfur-oxidizing Acidithiobacillus ferrooxidans

Abstract

Bibliographical note

Keywords

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