Cell shipment without a cold chain

Research output: Chapter in Book/Report/Conference proceedingConference proceedingpeer-review

Abstract

For transportation, cells are typically shipped in a cryopreserved state, either using liquid nitrogen or dry ice. These methods present a range of logistic challenges, are expensive and often require the use of the cytotoxic cryoprotectant DMSO.
LSG Ltd is working with Coventry University to develop a novel cell shipment medium (CellShip), designed for transporting and storing cells at ambient temperatures. We have identified a defined, xeno free formulation that maintains cell viability over a period of 72 h, including transport by a commercial courier.
HEK293 cells were cultured using complete MEM at 37°C in a humidified 5% CO2 atmosphere. For transportation, cells were dissociated and pelleted, the culture medium was discarded, and cells were washed once using CellShip. Cells were resuspended in CellShip at 1.4 – 2.3 x 106 cells per mL in 2 mL aliquots and shipped in cryovials using a commercial courier. Temperatures were monitored using temperature data loggers. Following the 72h transport/storage period, cells were counted and viability assessed, the cells were then recovered in complete MEM at 37°C in a humidified 5% CO2 atmosphere. Cell numbers and viability were assessed at 24 h and 48 h.
Following the 72h shipment/storage period the mean fold-change in viable cell number was 1.03 (n=4), with a mean cell viability of 98.8%, suggesting that cell number and viability had been maintained. Temperatures recorded ranged 180
from 11C – 27.4°C, with the maximum range of 13°C in a single experiment. Following 24h recovery, cells showed a fold-change of 1.72, which increased to 2.84 by 48h, indicating that there was no lag in recovery, which is often seen following cryopreservation.
A method for transporting cells at ambient temperatures would simplify the supply chain and provide a cost-effective, xeno-free, non-toxic alternative to cryopreservation, benefitting researchers and the cell-based therapy market.
Original languageEnglish
Title of host publicationCryoletters
PublisherIngenta Connect
Pages179-180
Number of pages2
Volume41
Edition3
Publication statusPublished - 5 Sep 2020
EventSLTB 2019 Meeting And Joint Workshop Organised By The Stem Cells User Group, Andalusian Initiative For Advanced Therapies And The Society For Low Temperature Biology - Sevilla, Spain
Duration: 2 Oct 20194 Oct 2019

Conference

ConferenceSLTB 2019 Meeting And Joint Workshop Organised By The Stem Cells User Group, Andalusian Initiative For Advanced Therapies And The Society For Low Temperature Biology
CountrySpain
CitySevilla
Period2/10/194/10/19

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