Candida albicans colonization and dissemination from the murine gastrointestinal tract: The influence of morphology and Th17 immunity

Simon Vautier, Rebecca A. Drummond, Kong Chen, Graeme I. Murray, David Kadosh, Alistair J.P. Brown, Neil A.R. Gow, Donna M. Maccallum, Jay K. Kolls, Gordon D. Brown

Research output: Contribution to journalArticle

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Abstract

The ability of Candida albicans to cause disease is associated with its capacity to undergo morphological transition between yeast and filamentous forms, but the role of morphology in colonization and dissemination from the gastrointestinal (GI) tract remains poorly defined. To explore this, we made use of wild-type and morphological mutants of C.albicans in an established model of GI tract colonization, induced following antibiotic treatment of mice. Our data reveal that GI tract colonization favours the yeast form of C.albicans, that there is constitutive low level systemic dissemination in colonized mice that occurs irrespective of fungal morphology, and that colonization is not controlled by Th17 immunity in otherwise immunocompetent animals. These data provide new insights into the mechanisms of pathogenesis and commensalism of C.albicans, and have implications for our understanding of human disease. Candida albicans is a commensal of the human gastrointestinal (GI) tract but can also spread from this site to cause systemic disease following immune perturbation. Here, using morphologically-locked strains we show that although the yeast form is favoured in the GI tract, both the yeast and hyphal forms can disseminate from this site to distal tissues in healthy animals. Finally, we show that Th17 immunity has no role in fungal colonisation or dissemination from the GI tract.

Original languageEnglish
Pages (from-to)445-450
Number of pages6
JournalCellular Microbiology
Volume17
Issue number4
Early online date25 Nov 2014
DOIs
Publication statusPublished - Apr 2015
Externally publishedYes

Fingerprint

Candida albicans
Gastrointestinal Tract
Immunity
Yeasts
Symbiosis
Immune System Diseases
Anti-Bacterial Agents

Bibliographical note

© 2014 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Candida albicans colonization and dissemination from the murine gastrointestinal tract : The influence of morphology and Th17 immunity. / Vautier, Simon; Drummond, Rebecca A.; Chen, Kong; Murray, Graeme I.; Kadosh, David; Brown, Alistair J.P.; Gow, Neil A.R.; Maccallum, Donna M.; Kolls, Jay K.; Brown, Gordon D.

In: Cellular Microbiology, Vol. 17, No. 4, 04.2015, p. 445-450.

Research output: Contribution to journalArticle

Vautier, S, Drummond, RA, Chen, K, Murray, GI, Kadosh, D, Brown, AJP, Gow, NAR, Maccallum, DM, Kolls, JK & Brown, GD 2015, 'Candida albicans colonization and dissemination from the murine gastrointestinal tract: The influence of morphology and Th17 immunity' Cellular Microbiology, vol. 17, no. 4, pp. 445-450. https://doi.org/10.1111/cmi.12388
Vautier, Simon ; Drummond, Rebecca A. ; Chen, Kong ; Murray, Graeme I. ; Kadosh, David ; Brown, Alistair J.P. ; Gow, Neil A.R. ; Maccallum, Donna M. ; Kolls, Jay K. ; Brown, Gordon D. / Candida albicans colonization and dissemination from the murine gastrointestinal tract : The influence of morphology and Th17 immunity. In: Cellular Microbiology. 2015 ; Vol. 17, No. 4. pp. 445-450.
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