Biosynthesis of Ag3PO4 nanoparticles in the absence of phosphate source using a phosphorus mineralising bacterium

Mohammadhosein Rahimi, Mohammad Raouf Hosseini, Mehran Bakhshi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Silver phosphate nanoparticles were biologically synthesised, for the first time, using a dilute silver nitrate solution as the silver ion supplier, and without any source of phosphate ion. The applied bacterium was Sporosarcina pasteurii formerly known as Bacillus pasteurii which is capable of solubilising phosphate from soils. It was speculated that the microbe accumulated phosphate from the organic source during the growth period, and then released it to deionised water. According to the transmission electron microscopy images and X-ray diffraction results, the produced nanoparticles were around 20 nm in size and identified as silver phosphate nanocrystals. The outcomes were also approved by energy-dispersive X-ray analysis, thermogravimetric and differential scanning calorimetry analyses, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy analysis. Finally, the antibacterial effect of the obtained nanoparticles was verified by testing them against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium. The activity of silver phosphate nanoparticles against gram-negative strains was better than the gram positives. It should be mentioned that the concentrations of 500 and 1000 mg/l were found to be strongly inhibitory for all of the strains.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalIET Nanobiotechnology
Volume12
Issue number1
Early online date23 Oct 2017
DOIs
Publication statusPublished - 1 Feb 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© The Institution of Engineering and Technology 2017.

Keywords

  • nanoparticles
  • silver compounds
  • nanofabrication
  • microorganisms
  • antibacterial activity
  • transmission electron microscopy
  • X-ray diffraction
  • X-ray chemical analysis
  • differential scanning calorimetry
  • ultraviolet spectra
  • visible spectra
  • Fourier transform infrared spectra
  • biosynthesis
  • phosphate source
  • phosphorus mineralising bacterium
  • silver phosphate nanoparticles
  • Sporosarcina pasteurii
  • Bacillus pasteurii
  • deionised water
  • transmission electron microscopy images
  • energy-dispersive X-ray analysis
  • thermogravimetric analyses
  • differential scanning calorimetry analyses
  • ultraviolet–visible spectroscopy
  • Fourier transform infrared spectroscopy
  • antibacterial effect
  • Bacillus cereus
  • Staphylococcus aureus
  • Escherichia coli
  • Salmonella typhimurium
  • Ag3 PO4

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biotechnology
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'Biosynthesis of Ag3PO4 nanoparticles in the absence of phosphate source using a phosphorus mineralising bacterium'. Together they form a unique fingerprint.

Cite this