Bacterial and Archaeal Diversity in Sulfide-Bearing Waste Rock at Faro Mine Complex, Yukon Territory, Canada

Eva Pakostova, D. Barrie Johnson, Zhongwen Bao, Peter M. MacKenzie, Carol J. Ptacek, David W. Blowes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Acid mine/rock drainage (AMD/ARD) is generated by the microbially-accelerated oxidative dissolution of sulfide minerals in working and abandoned mine sites, and some natural environments. Iron-oxidizing microorganisms (IOM) regenerate the oxidant Fe3+, while sulfur-oxidizing microorganisms (SOM) contribute to AMD/ARD by generating H2SO4 via the oxidation of elemental S and reduced inorganic sulfur compounds. Bacterial and archaeal diversity in 34 samples of sulfide-bearing waste rock recovered from three boreholes at the Faro Mine Complex (Yukon Territory, Canada; Pb/Zn production from 1969 to 1998) was investigated using high-throughput amplicon sequencing of 16S rRNA genes. A majority of the borehole pore water samples had circum-neutral or mildly alkaline pH (6.38.7), while some had low pH (3.35.2). Mean relative abundance of prokaryotic SOM/IOM accounted for 3.4% of the total amplicons. The acidophilic genera Alicyclobacillus and Acidithiobacillus were the most abundant sulfur- and iron-metabolizing prokaryotes detected, followed by neutrophilic and moderately acidophilic (non-iron-oxidizing) SOM. Sulfate-reducing bacteria (SRB) were also detected, accounting for 0.6% of total reads. The presence of both acidophilic and neutrophilic prokaryotes catalyzing transformations of sulfur and iron in the same samples suggests development of microenvironments within the waste rock where dynamic biogeochemical transformations of these elements occur.
Original languageEnglish
Pages (from-to)511–519
Number of pages9
JournalGeomicrobiology Journal
Volume37
Issue number6
Early online date26 Feb 2020
DOIs
Publication statusPublished - 1 Jun 2020
Externally publishedYes

Bibliographical note

This is an Accepted Manuscript of an article published by Taylor & Francis in Geomicrobiology Journal, on 26/02/2020 available online: http://www.tandfonline.com/10.1080/01490451.2020.1731020

Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

Keywords

  • Acid mine drainage
  • iron-oxidizers
  • mine waste
  • sulfur-oxidizers
  • waste-rock dump

ASJC Scopus subject areas

  • Microbiology
  • Environmental Chemistry
  • Environmental Science(all)
  • Earth and Planetary Sciences (miscellaneous)

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