Assessing the effects of exposure to environmental stress on some functional properties of Bifidobacterium animalis ssp. lactis

Daniel Amund, L. I. I. Ouoba, J. P. Sutherland, H. B. Ghoddusi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study assessed the effects of exposing a strain of Bifidobacterium animalis ssp. lactis to acid, bile and osmotic stresses on antagonistic properties, biofilm formation and antibiotic susceptibility/resistance profile. Exposure to each stress factor appeared to have no significant effect on the antagonism against Escherichia coli NCTC 12900 and Salmonella enterica serovar Enteritidis PT4. No suppression in biofilm formation due to exposure to stress was observed. Bile and osmotic stresses resulted in significantly higher biofilm formation. Expression of an exopolysaccharide synthesis gene, gtf 01207, was significantly higher when the B. animalis ssp. lactis strain was exposed to osmotic stress. Susceptibility of the B. animalis ssp. lactis strain to chloramphenicol, erythromycin, ampicillin and vancomycin, and resistance to tetracycline remained unchanged when exposed to each stress. The expression of a tetracycline resistance gene, tet(W), was significantly higher when exposed to each stress. These results may suggest that the potential for the B. animalis ssp. lactis strain to provide probiotic benefit, after exposure to the stressful conditions of the gastrointestinal tract, remains intact.
Original languageEnglish
Pages (from-to)461-469
JournalBeneficial microbes
Volume5
Issue number4
Early online date5 Aug 2014
DOIs
Publication statusPublished - 2014

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Environmental Exposure
Osmotic Pressure
Biofilms
Vancomycin Resistance
Ampicillin Resistance
Tetracycline Resistance
Salmonella enteritidis
Probiotics
Chloramphenicol
Erythromycin
Microbial Drug Resistance
Tetracycline
Bile Acids and Salts
Bile
Genes
Gastrointestinal Tract
Escherichia coli
Bifidobacterium animalis

Bibliographical note

The full text is currently unavailable on the repository.

Keywords

  • probiotics
  • antimicrobial activity
  • biofilm
  • antibiotic susceptibility
  • gastrointestinal stress

Cite this

Assessing the effects of exposure to environmental stress on some functional properties of Bifidobacterium animalis ssp. lactis. / Amund, Daniel; Ouoba, L. I. I.; Sutherland, J. P.; Ghoddusi, H. B.

In: Beneficial microbes, Vol. 5, No. 4, 2014, p. 461-469.

Research output: Contribution to journalArticle

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