Ascorbyl palmitate/DSPE-PEG nanocarriers for oral iron delivery: Preparation, characterisation and in vitro evaluation

M Gulrez Zariwala, Sebastien Farnaud, Zahra Merchant, Satyanarayana Somavarapu, Derek Renshaw

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption.

Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalColloids and surfaces. B, Biointerfaces
Volume115
Early online date23 Nov 2013
DOIs
Publication statusPublished - 1 Mar 2014
Externally publishedYes

Fingerprint

Ascorbyl palmitate
Polyethylene glycols
glycols
polyethylenes
delivery
Chitosan
Iron
iron
preparation
ferrous sulfate
evaluation
entrapment
blanks
proteins
cells
Proteins
Particle Size
sulfates
Particle size
inclusions

Keywords

  • Absorption
  • Administration, Oral
  • Ascorbic Acid
  • Caco-2 Cells
  • Cell Death
  • Cell Survival
  • Drug Carriers
  • Drug Delivery Systems
  • Humans
  • Iron
  • Nanoparticles
  • Particle Size
  • Phosphatidylethanolamines
  • Polyethylene Glycols
  • Static Electricity
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Ascorbyl palmitate
  • Ferrous sulphate
  • Caco-2
  • Chitosan
  • Nanocarriers
  • Fortification

Cite this

Ascorbyl palmitate/DSPE-PEG nanocarriers for oral iron delivery : Preparation, characterisation and in vitro evaluation. / Zariwala, M Gulrez; Farnaud, Sebastien; Merchant, Zahra; Somavarapu, Satyanarayana; Renshaw, Derek.

In: Colloids and surfaces. B, Biointerfaces, Vol. 115, 01.03.2014, p. 86-92.

Research output: Contribution to journalArticle

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T2 - Preparation, characterisation and in vitro evaluation

AU - Zariwala, M Gulrez

AU - Farnaud, Sebastien

AU - Merchant, Zahra

AU - Somavarapu, Satyanarayana

AU - Renshaw, Derek

N1 - Copyright © 2013 Elsevier B.V. All rights reserved.

PY - 2014/3/1

Y1 - 2014/3/1

N2 - The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption.

AB - The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption.

KW - Absorption

KW - Administration, Oral

KW - Ascorbic Acid

KW - Caco-2 Cells

KW - Cell Death

KW - Cell Survival

KW - Drug Carriers

KW - Drug Delivery Systems

KW - Humans

KW - Iron

KW - Nanoparticles

KW - Particle Size

KW - Phosphatidylethanolamines

KW - Polyethylene Glycols

KW - Static Electricity

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Ascorbyl palmitate

KW - Ferrous sulphate

KW - Caco-2

KW - Chitosan

KW - Nanocarriers

KW - Fortification

U2 - 10.1016/j.colsurfb.2013.11.028

DO - 10.1016/j.colsurfb.2013.11.028

M3 - Article

VL - 115

SP - 86

EP - 92

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -