Abstract
The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption.
Original language | English |
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Pages (from-to) | 86-92 |
Number of pages | 7 |
Journal | Colloids and surfaces. B, Biointerfaces |
Volume | 115 |
Early online date | 23 Nov 2013 |
DOIs | |
Publication status | Published - 1 Mar 2014 |
Externally published | Yes |
Keywords
- Absorption
- Administration, Oral
- Ascorbic Acid
- Caco-2 Cells
- Cell Death
- Cell Survival
- Drug Carriers
- Drug Delivery Systems
- Humans
- Iron
- Nanoparticles
- Particle Size
- Phosphatidylethanolamines
- Polyethylene Glycols
- Static Electricity
- Journal Article
- Research Support, Non-U.S. Gov't
- Ascorbyl palmitate
- Ferrous sulphate
- Caco-2
- Chitosan
- Nanocarriers
- Fortification
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Profiles
-
Sebastien Farnaud
- Centre for Health and Life Sciences - Professor of Enterprise and Innovation in Healthcare Technology
Person: Teaching and Research
-
Derek Renshaw, Centre Director
- Centre for Health and Life Sciences - Centre Director
Person: Professional Services