Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers

Christophe Lambing, Kim Osman, Komsun Nuntasoontorn, Allan West, James D Higgins, Gregory P Copenhaver, Jianhua Yang, Susan J Armstrong, Karl Mechtler, Elisabeth Roitinger, F Chris H Franklin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has revealed dynamic changes in the chromosome axis in Arabidopsis thaliana and Brassica oleracea. We show that the axis associated protein ASY1 is depleted during zygotene concomitant with synaptonemal complex (SC) formation. Study of an Atpch2 mutant demonstrates this requires the conserved AAA+ ATPase, PCH2, which localizes to the sites of axis remodelling. Loss of PCH2 leads to a failure to deplete ASY1 from the axes and compromizes SC polymerisation. Immunolocalization of recombination proteins in Atpch2 indicates that recombination initiation and CO designation during early prophase I occur normally. Evidence suggests that CO interference is initially functional in the mutant but there is a defect in CO maturation following designation. This leads to a reduction in COs and a failure to form COs between some homologous chromosome pairs leading to univalent chromosomes at metaphase I. Genetic analysis reveals that CO distribution is also affected in some chromosome regions. Together these data indicate that the axis remodelling defect in Atpch2 disrupts normal patterned formation of COs.

Original languageEnglish
Article numbere1005372
JournalPLoS Genetics
Volume11
Issue number7
DOIs
Publication statusPublished - 16 Jul 2015
Externally publishedYes

Fingerprint

Arabidopsis
maturation
chromosome
Carbon Monoxide
Chromosomes
chromosomes
Synaptonemal Complex
Meiotic Prophase I
synaptonemal complex
prophase
Genetic Recombination
recombination
protein
defect
Protein Array Analysis
mutants
proteins
Brassica
Meiosis
genetic analysis

Keywords

  • Adenosine Triphosphatases
  • Arabidopsis
  • Arabidopsis Proteins
  • Chromatin Assembly and Disassembly
  • Chromosomes, Plant
  • Crossing Over, Genetic
  • DNA-Binding Proteins
  • Meiosis
  • Synaptonemal Complex
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Lambing, C., Osman, K., Nuntasoontorn, K., West, A., Higgins, J. D., Copenhaver, G. P., ... Franklin, F. C. H. (2015). Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers. PLoS Genetics, 11(7), [e1005372]. https://doi.org/10.1371/journal.pgen.1005372

Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers. / Lambing, Christophe; Osman, Kim; Nuntasoontorn, Komsun; West, Allan; Higgins, James D; Copenhaver, Gregory P; Yang, Jianhua; Armstrong, Susan J; Mechtler, Karl; Roitinger, Elisabeth; Franklin, F Chris H.

In: PLoS Genetics, Vol. 11, No. 7, e1005372, 16.07.2015.

Research output: Contribution to journalArticle

Lambing, C, Osman, K, Nuntasoontorn, K, West, A, Higgins, JD, Copenhaver, GP, Yang, J, Armstrong, SJ, Mechtler, K, Roitinger, E & Franklin, FCH 2015, 'Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers' PLoS Genetics, vol. 11, no. 7, e1005372. https://doi.org/10.1371/journal.pgen.1005372
Lambing C, Osman K, Nuntasoontorn K, West A, Higgins JD, Copenhaver GP et al. Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers. PLoS Genetics. 2015 Jul 16;11(7). e1005372. https://doi.org/10.1371/journal.pgen.1005372
Lambing, Christophe ; Osman, Kim ; Nuntasoontorn, Komsun ; West, Allan ; Higgins, James D ; Copenhaver, Gregory P ; Yang, Jianhua ; Armstrong, Susan J ; Mechtler, Karl ; Roitinger, Elisabeth ; Franklin, F Chris H. / Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers. In: PLoS Genetics. 2015 ; Vol. 11, No. 7.
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