Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function

Yashar Sadigh; Na  Tang; Yashar  Sadigh; Susan  Baigent; Zhiqiang  Shen; Venugopal  Nair; Yongxiu  Yao

doi:10.3390/v10060279

Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function

Yashar Sadigh, Na Tang, Yashar Sadigh , Susan Baigent, Zhiqiang Shen, Venugopal Nair , Yongxiu Yao

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Abstract

Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek’s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis

Original language	English
Number of pages	12
Journal	Viruses
Volume	10
Issue number	6
DOIs	https://doi.org/10.3390/v10060279
Publication status	Published - 24 May 2018
Externally published	Yes

Bibliographical note

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

CRISPR
Cas9
CVI988
Meq; pp38;
deletion

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v10060279

PublishedFinal published version, 1.8 MBLicence: CC BY

Cite this

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title = "Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function",

abstract = "Marek{\textquoteright}s disease virus (MDV) is a member of alphaherpesviruses associated with Marek{\textquoteright}s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek{\textquoteright}s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis",

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author = "Yashar Sadigh and Na Tang and Yashar Sadigh and Susan Baigent and Zhiqiang Shen and Venugopal Nair and Yongxiu Yao",

note = "{\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).",

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month = may,

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doi = "10.3390/v10060279",

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AU - Sadigh, Yashar

AU - Tang, Na

AU - Sadigh , Yashar

AU - Baigent, Susan

AU - Shen, Zhiqiang

AU - Nair , Venugopal

AU - Yao , Yongxiu

N1 - © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PY - 2018/5/24

Y1 - 2018/5/24

N2 - Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek’s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis

AB - Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek’s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis

KW - CRISPR

KW - Cas9

KW - CVI988

KW - Meq; pp38;

KW - deletion

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U2 - 10.3390/v10060279

DO - 10.3390/v10060279

M3 - Article

SN - 1999-4915

VL - 10

JO - Viruses

JF - Viruses

IS - 6

ER -

Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function

Abstract

Bibliographical note

Keywords

UN SDGs

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