An investigation into the B lymphopoietic capacity of long-term bone marrow cultures

R. Aspinall, J. J.T. Owen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A suspension of nucleated femoral (BALB/c x CBA) bone marrow cells was inoculated, either into glass flasks or into flasks coated with a preformed syngeneic marrow derived monolayer, and cultured for long periods in media supplemented with hydrocortisone sodium succinate. Long term analysis of the lymphoid status of these cultures was then carried out whilst they were producing CFU-S cells. The results show that T cells expressing the Thy 1 marker were present for up to 10 weeks, surface immunoglobulin positive (sIgM+) B cells were present for at least 6 weeks, whereas pre-B cells were present for no longer than one week. Pre-B, B and T cells were all present at levels well below those found in normal healthy bone marrow. When cultures were free of both B and pre-B lymphocytes, the cells collected from them were used to reconstitute lethally irradiated mice. Reconstitution was of the type (A x B)→A and approximately 2 months elapsed before reconstituted animals were analysed.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalImmunology
Volume48
Issue number1
Publication statusPublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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