Adverse Effects of High-Fat Diet Consumption on Contractile Mechanics of Isolated Mouse Skeletal Muscle are Reduced When Supplemented With Resveratrol

Increasing evidence indicates resveratrol (RES) supplementation evokes anti-obesogenic responses which could mitigate obesity-induced reductions in skeletal muscle (SkM) contractility. Contractile function is a key facet of SkM health that underpins whole body health, and the present study will for the first time examine effects of high-fat diet and RES supplementation on isolated soleus (SOL) and extensor digitorum longus (EDL) contractile function. Female CD-1 mice, ~6-weeks old (N=38), consumed standard (SLD) or HFD, with or without RES (4g/kg-1 diet) for 12-weeks. SOL and EDL (n=8-10 per muscle, per group) were isolated and absolute and normalised (to muscle size and body mass) isometric force and work loop power output (PO) measured, and fatigue resistance determined. Furthermore, sirtuin-1 expression was determined to provide mechanistic insight into any potential contractile changes. For SOL absolute force was higher in HFDRES compared to HFD (P=0.033), and PO normalised to body mass and cumulative work during fatigue were reduced in HFD groups (P<0.014). EDL absolute and normalised PO and cumulative work during fatigue was lower in HFD compared to other groups (P<0.019). RES negated most adverse effects of HFD consumption on EDL contractility, with HFDRES producing comparable PO and cumulative work as SLD groups. Sirtuin-1 expression was not influenced by diet in either muscle (P>0.165). This study uniquely demonstrates that RES attenuates HFD-induced reductions in contractile performance of EDL, but this response is not explained by altered Sirtuin-1 expression. These results suggest RES may be an appropriate strategy to alleviate obesity-induced declines in SkM function.