TY - GEN
T1 - Adsorbed on the adsorbed protein layer formed on a laser modified zirconia bioceramic
AU - Hao, Liang
AU - Lawrence, Jonathan
PY - 2004/12/1
Y1 - 2004/12/1
N2 - The nature of the surface strongly influences the composition and recognisability of the adsorbed protein layer, which in turn affects the subsequent cellular interactions. Thus, to understand the biological response to a material, especially in vitro, one must fully understand the nature of the adsorbed protein film that forms on the material. This study is to investigate the fundamental interactions between the human serum albumin (no-cell adhesive) and human plasma fibronectin and bioinert ceramic following the CO2 laser treatment. The analysis of the albumin and fibronectin adsorption was conducted on the untreated and CO2 laser modified magnesia partially stabilised zirconia (MgO-PSZ) bioceramic using an ellipsometry. It was found that the adsorptions of albumin and fibronectin were influenced by the surface properties. The albumin adsorption was affected by the surface roughness and wettability characteristics of the MgOPSZ and decreased with these properties. While the fibronectin adsorption was increased with wettability characteristic and predominantly governed by this property. Moreover, the considerable change in the polar component of surface energy,γ svp, and its effect on protein adsorption implied that the albumin and fibronectin adsorption on the MgO-PSZ surfaces was probably due to the polar and chemical interactions. The value of this work is to provide a novel technique and useful information for mediating protein adsorption and thereof cellular interactions.
AB - The nature of the surface strongly influences the composition and recognisability of the adsorbed protein layer, which in turn affects the subsequent cellular interactions. Thus, to understand the biological response to a material, especially in vitro, one must fully understand the nature of the adsorbed protein film that forms on the material. This study is to investigate the fundamental interactions between the human serum albumin (no-cell adhesive) and human plasma fibronectin and bioinert ceramic following the CO2 laser treatment. The analysis of the albumin and fibronectin adsorption was conducted on the untreated and CO2 laser modified magnesia partially stabilised zirconia (MgO-PSZ) bioceramic using an ellipsometry. It was found that the adsorptions of albumin and fibronectin were influenced by the surface properties. The albumin adsorption was affected by the surface roughness and wettability characteristics of the MgOPSZ and decreased with these properties. While the fibronectin adsorption was increased with wettability characteristic and predominantly governed by this property. Moreover, the considerable change in the polar component of surface energy,γ svp, and its effect on protein adsorption implied that the albumin and fibronectin adsorption on the MgO-PSZ surfaces was probably due to the polar and chemical interactions. The value of this work is to provide a novel technique and useful information for mediating protein adsorption and thereof cellular interactions.
UR - http://www.scopus.com/inward/record.url?scp=56749143060&partnerID=8YFLogxK
M3 - Conference proceeding
SN - 0912035773
SN - 9780912035772
T3 - ICALEO 2004 - 23rd International Congress on Applications of Laser and Electro-Optics, Congress Proceedings
BT - Proceedings of the 23rd international congress on applications of lasers and electro-optics 2004
A2 - Patel, Rajesh
PB - Laser Institute of America
T2 - 23rd International Congress on Applications of Laser and Electro-Optics
Y2 - 4 October 2004 through 7 October 2004
ER -