Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

Linlin Qi, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hoermayer, Scott Sinclair, Minxia Zou, Charo del Genio, Martin Kubeš, Richard Napier, Krzysztof Jaworski, Jiří Friml

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)
546 Downloads (Pure)

Abstract

The phytohormone auxin is the major coordinative signal in plant development, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.
Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalNature
Volume611
Early online date26 Oct 2022
DOIs
Publication statusPublished - 3 Nov 2022

Bibliographical note

Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

This document is the author’s post-print version, incorporating any revisions agreed during the peer-review process. Some differences between the published version and this version may remain and you are advised to consult the published version if you wish to cite from it.

Funder


Funding Information: This research was supported by the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) of IST Austria. We thank C. Gehring for suggestions and advice; and K. U. Torii and G. Stacey for seeds and plasmids. This project was funded by a European Research Council Advanced Grant (ETAP-742985). M.F.K. and R.N. acknowledge the support of the EU MSCA-IF project CrysPINs (792329). M.K. was supported by the project POWR.03.05.00-00-Z302/17 Universitas Copernicana Thoruniensis in Futuro–IDS “Academia Copernicana”. CIDG acknowledges support from UKRI under Future Leaders Fellowship grant number MR/T020652/1. .

Keywords

  • Adenylyl Cyclases - genetics - metabolism
  • Arabidopsis - enzymology - metabolism
  • Arabidopsis Proteins - genetics - metabolism
  • Cyclic AMP - metabolism
  • F-Box Proteins - genetics - metabolism
  • Gene Expression Regulation, Plant
  • Gravitropism
  • Indoleacetic Acids - metabolism - pharmacology
  • Mutation
  • Plant Growth Regulators - pharmacology - metabolism
  • Plant Roots - growth & development
  • Receptors, Cell Surface - genetics - metabolism
  • Second Messenger Systems

Fingerprint

Dive into the research topics of 'Adenylate cyclase activity of TIR1/AFB auxin receptors in plants'. Together they form a unique fingerprint.

Cite this