A simple method for the measurement of sjTREC levels in blood

Richard Aspinall, Jeff Pido, Deborah Andrew

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalMechanisms of Ageing and Development
Issue number1-3
Publication statusPublished - 20 Jan 2001
Externally publishedYes


  • PCR
  • sjTREC
  • Thymic output

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology


Dive into the research topics of 'A simple method for the measurement of sjTREC levels in blood'. Together they form a unique fingerprint.

Cite this