A simple method for the measurement of sjTREC levels in blood

Richard Aspinall; Jeff Pido; Deborah Andrew

doi:10.1016/S0047-6374(00)00197-4

A simple method for the measurement of sjTREC levels in blood

Richard Aspinall, Jeff Pido, Deborah Andrew

Chelsea and Westminister Hospital NHS Foundation Trust

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.

Original language	English
Pages (from-to)	59-67
Number of pages	9
Journal	Mechanisms of Ageing and Development
Volume	121
Issue number	1-3
DOIs	https://doi.org/10.1016/S0047-6374(00)00197-4
Publication status	Published - 20 Jan 2001
Externally published	Yes

Keywords

PCR
sjTREC
Thymic output

ASJC Scopus subject areas

Ageing
Developmental Biology

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10.1016/S0047-6374(00)00197-4

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abstract = "We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.",

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N2 - We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.

AB - We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.

KW - PCR

KW - sjTREC

KW - Thymic output

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ER -

A simple method for the measurement of sjTREC levels in blood

Abstract

Keywords

ASJC Scopus subject areas

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