A simple method for the measurement of sjTREC levels in blood

Richard Aspinall, Jeff Pido, Deborah Andrew

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalMechanisms of Ageing and Development
Volume121
Issue number1-3
DOIs
Publication statusPublished - 20 Jan 2001
Externally publishedYes

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Keywords

  • PCR
  • sjTREC
  • Thymic output

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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