A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells

Jaap Kampinga, Frans G.M. Kroese, Gerda H. Pol, Paul Nieuwenhuis, Friedrich Haag, Prim B. Singh, Bruce Roser, Richard Aspinall

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A hybridoma producing the monoclonal antibody HIS42 was isolated from a fusion between spleen cells from a BALB/c mouse immunized with rat thymocytes and the fusion partner SP2/0. This antibody recognizes a minor subset of T cells in every haplotype of rat tested so tar. The subpopulation of HIS42 positive T cells contains both CD4+ and CD8+ cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS42 induces T cell proliteration in the presence of Interleukin-2. In contrast to lymph node T cells, a number of thymocytes were found to express HIS42 only in the cytoplasm or together with membrane expression. Most bright HIS42 surface labelled thymocytes were also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS-PAGE analyses of the membrane molecules lmmunoprecipitated by HIS42 show two bands on unreduced gels. One of these bands (85 kd) runs as two separate bands at 35 and 48 kd on reduction. The other much weaker broad band (̃100 kd) is hardly affected by reduced conditions. Taken together these data suggest that HIS42 is directed against a determinant on the rat T cell receptor for antigen, which is common to a small number of T cells.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalInternational Immunology
Volume1
Issue number3
DOIs
Publication statusPublished - 1 Jul 1989
Externally publishedYes

Keywords

  • , Vβ family
  • Monoclonal antibody
  • Rat
  • T cell antigen receptor
  • T cell proliferation
  • Thymocyte differentiation

ASJC Scopus subject areas

  • Immunology

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