A case of transplacental transmission of Theileria equi in a foal in Trinidad

Karla C. Georges; Chuckwudozi D. Ezeokoli; Olivier Sparagano; Indira Pargass; Mervyn Campbell; Roger D'Abadie; Michael J. Yabsley

doi:10.1016/j.vetpar.2010.10.019

A case of transplacental transmission of Theileria equi in a foal in Trinidad

Karla C. Georges, Chuckwudozi D. Ezeokoli, Olivier Sparagano, Indira Pargass, Mervyn Campbell, Roger D'Abadie, Michael J. Yabsley

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Equine piroplasmosis due to Theileria equi and Babesia caballi is endemic in Trinidad. A case of equine piroplasmosis due to T. equi was diagnosed in a thoroughbred foal at 10h post-partum. A high parasitaemia (63%) of piroplasms was observed in a Wright-Giemsa ^® stained thin blood smear from the foal. In addition, the 18S rRNA gene for Babesia/Theileria was amplified from DNA extracted from the blood of the foal and the mare. Amplified products were subjected to a reverse line blot hybridization assay (RLB), which confirmed the presence of T. equi DNA in the foal. The mare was negative by RLB but was positive for T. equi using a nested PCR and sequence analysis. In areas where equine piroplasmosis is endemic, severe jaundice in a post-partum foal may be easily misdiagnosed as neonatal isoerythrolysis. Foals with post-partum jaundice should be screened for equine piroplasmosis, which may be confirmed using molecular methods if available.

Original language	English
Pages (from-to)	363-366
Number of pages	4
Journal	Veterinary Parasitology
Volume	175
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetpar.2010.10.019
Publication status	Published - 10 Feb 2011
Externally published	Yes

Keywords

Equine piroplasmosis
Neonatal foals
Reverse line blot
Trinidad

ASJC Scopus subject areas

Parasitology
veterinary(all)

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10.1016/j.vetpar.2010.10.019

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title = "A case of transplacental transmission of Theileria equi in a foal in Trinidad",

abstract = "Equine piroplasmosis due to Theileria equi and Babesia caballi is endemic in Trinidad. A case of equine piroplasmosis due to T. equi was diagnosed in a thoroughbred foal at 10h post-partum. A high parasitaemia (63%) of piroplasms was observed in a Wright-Giemsa {\textregistered} stained thin blood smear from the foal. In addition, the 18S rRNA gene for Babesia/Theileria was amplified from DNA extracted from the blood of the foal and the mare. Amplified products were subjected to a reverse line blot hybridization assay (RLB), which confirmed the presence of T. equi DNA in the foal. The mare was negative by RLB but was positive for T. equi using a nested PCR and sequence analysis. In areas where equine piroplasmosis is endemic, severe jaundice in a post-partum foal may be easily misdiagnosed as neonatal isoerythrolysis. Foals with post-partum jaundice should be screened for equine piroplasmosis, which may be confirmed using molecular methods if available.",

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AU - Georges, Karla C.

AU - Ezeokoli, Chuckwudozi D.

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AU - Pargass, Indira

AU - Campbell, Mervyn

AU - D'Abadie, Roger

AU - Yabsley, Michael J.

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N2 - Equine piroplasmosis due to Theileria equi and Babesia caballi is endemic in Trinidad. A case of equine piroplasmosis due to T. equi was diagnosed in a thoroughbred foal at 10h post-partum. A high parasitaemia (63%) of piroplasms was observed in a Wright-Giemsa ® stained thin blood smear from the foal. In addition, the 18S rRNA gene for Babesia/Theileria was amplified from DNA extracted from the blood of the foal and the mare. Amplified products were subjected to a reverse line blot hybridization assay (RLB), which confirmed the presence of T. equi DNA in the foal. The mare was negative by RLB but was positive for T. equi using a nested PCR and sequence analysis. In areas where equine piroplasmosis is endemic, severe jaundice in a post-partum foal may be easily misdiagnosed as neonatal isoerythrolysis. Foals with post-partum jaundice should be screened for equine piroplasmosis, which may be confirmed using molecular methods if available.

AB - Equine piroplasmosis due to Theileria equi and Babesia caballi is endemic in Trinidad. A case of equine piroplasmosis due to T. equi was diagnosed in a thoroughbred foal at 10h post-partum. A high parasitaemia (63%) of piroplasms was observed in a Wright-Giemsa ® stained thin blood smear from the foal. In addition, the 18S rRNA gene for Babesia/Theileria was amplified from DNA extracted from the blood of the foal and the mare. Amplified products were subjected to a reverse line blot hybridization assay (RLB), which confirmed the presence of T. equi DNA in the foal. The mare was negative by RLB but was positive for T. equi using a nested PCR and sequence analysis. In areas where equine piroplasmosis is endemic, severe jaundice in a post-partum foal may be easily misdiagnosed as neonatal isoerythrolysis. Foals with post-partum jaundice should be screened for equine piroplasmosis, which may be confirmed using molecular methods if available.

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A case of transplacental transmission of Theileria equi in a foal in Trinidad

Abstract

Keywords

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